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Plasmid pBR322 has PstI restriction enzyme site within gene \({\mathrm{amp}}^{\mathrm{R}}\) that confers ampicillin resi…

Q1

Plasmid pBR322 has PstI restriction enzyme site within gene \({\mathrm{amp}}^{\mathrm{R}}\) that confers ampicillin resistance, If this enzyme is used for inserting a gene for \(\beta\)-galactoside production and the recombinant plasmid is inserted in an E.coli strain

a

The transformed cells will have the ability to resist ampicillin as well as produce \(\beta\)-galactoside.

b

It will lead to lysis of host cell.

c

It will be able to produce a novel protein with dual ability.

d

It will not be able to confer ampicillin resistance to host cell.

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