Biotechnology - Principles and Processes

Which of the following statements is incorrect?

[NEET 2024]

Identify the incorrect statement related to gel electrophoresis.

[Re-NEET 2024]

The "Ti plasmid" of Agrobacterium tumefaciens stands for

[NEET 2024]

In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:

[Re-NEET 2024]

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

[NEET 2024]

Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification ( \(~1\) billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension

Choose the correct sequence of steps of PCR from the options given below :

[Re-NEET 2024]

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.

Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells.

Reason R: Isolation of genetic material needs disruption of cells.

In the light of the above statements, choose the most appropriate answer from the options given below :

[Re-NEET 2026]

Which of the following statements is incorrect?

[NEET 2024]

In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:

[Re-NEET 2024]

The "Ti plasmid" of Agrobacterium tumefaciens stands for

[NEET 2024]

Given below are two statements:

Statement I: Plasmids are autonomously replicating DNA.

Statement II: Plasmids are extrachromosomal DNA.

In the light of the above statements, choose the most appropriate answer from the options given below:

[Re-NEET 2026]

Which of the following are correct about EcoRI?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognises a specific palindromic sequence
D. Cut the DNA between the base G and A where it encounters the DNA sequence 'GAATTC'
E. Exonuclease

Choose the correct answer from the options given below:

[Re-NEET 2024]

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.

Choose the correct answer from the options given below:

[NEET 2024]

Which of the following statements are correct with respect to DNA separation, isolation and visualization?

A. The cutting of DNA is done by molecular scissors.

B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis.

C. The separated DNA fragments can be seen without staining when exposed to UV light.

D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light.

Choose the correct answer from the options given below :

[NEET 2026]

Identify the incorrect statement related to gel electrophoresis.

[Re-NEET 2024]

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :

[NEET 2026]

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (\(te{t}^{R}\) ) gene in the pBR322 cloning vector.

[Re-NEET 2024]

Which of the following statements are not true regarding restriction endonucleases?
A. They are called molecular scissors.
B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli.
C. They cut the DNA only at the centre of the palindromic sites.
D. They remove nucleotides only from the ends of DNA fragments.
E. They recognise specific palindromic base-pair sequences.
Choose the answer from the options given below :

[NEET 2026]

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

[NEET 2024]

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

[NEET 2024]

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.

Choose the correct answer from the options given below:

[NEET 2024]

Recombinant DNA molecule can be created normally by cutting the vector DNA and target DNA respectively with:

[Re-NEET 2024]

Insertion of a foreign DNA at BamHI site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards:

[NEET 2026]

Which of the following are correct about EcoR1?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognises a specific palindromic sequence
D. Cut the DNA between the base G and A wher encounters the DNA sequence 'GAATTC
E. Exonuclease

Choose the correct answer from the options giver below:

[Re-NEET 2024]

Polymerase chain reaction (PCR) amplifies DNA following the equation

[NEET 2025]

During PCR, primers bind to the DNA strands in the _____ step.

[Re-NEET 2026]

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (\(te{t}^{R}\) ) gene in the pBR322 cloning vector.

[Re-NEET 2024]

Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification ( \(~1\) billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension

Choose the correct sequence of steps of PCR from the options given below :

[Re-NEET 2024]

Recombinant DNA molecule can be created normally by cutting the vector DNA and target DNA respectively with:

[Re-NEET 2024]

Which of the following are correct about EcoR1?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognises a specific palindromic sequence
D. Cut the DNA between the base G and A wher encounters the DNA sequence 'GAATTC
E. Exonuclease

Choose the correct answer from the options giver below:

[Re-NEET 2024]

In gene gun method used to introduce alien DNA into host cells, microparticles of _______ metal are used.

[NEET 2023]

Identify the wrong statement with regard to restriction enzymes.

Upon exposure to UV radiation, DNA stained with ethidium bromide will show;

[NEET 2023]

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

[NEET 2021]

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

[NEET 2021]

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

[NEET 2023]

Select the correct statement from the following:

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of \(\mathrm{pBR}322\) :

In gel electrophoresis, separated DNA fragments can be visualized with the help of

[NEET 2020]

In gel electrophoresis, separated DNA fragments can be visualized with the help of:

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

In a mixture, DNA fragments are separated by:

[Re-NEET 2020]

The specific palindromic sequence which is recognized by EcoRI is

[NEET 2020]

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence

A. Insertion of recombinant DNA into the host cell.

B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment.

D. Amplification of gene of interest using PCR.

Choose the correct answer from the option given below:

In recombinant DNA technology antibiotics are used:

Choose the correct pair from the following:

Main steps in the formation of recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA from the cell.
D. Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:

[NEET 2023]

First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is

[Re-NEET 2020]

Which of the following is not a cloning vector?

Plasmid pBR322 has PstI restriction enzyme site within gene \({\mathrm{amp}}^{\mathrm{R}}\) that confers ampicillin resistance, If this enzyme is used for inserting a gene for \(\beta\)-galactoside production and the recombinant plasmid is inserted in an E.coli strain

Spooling is

[Re-NEET 2020]

Which of the following is not a cloning vector?

[NEET 2023]

Match List-I with List-II.

Choose the correct answer from the options given below:

Match the organism with its use in biotechnology.

Select the correct option from the following

Thermostable DNA polymerase used in PCR was isolated from:

Match the following techniques or instruments with their usage:

Select the correct option from following:
(1) (2) (3) (4)

The sequence that controls the copy number of the linked DNA in the vector, is termed:

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as

[NEET 2021]

In recombinant DNA technology antibiotics are used:

[Re-NEET 2020]

In gene gun method used to introduce alien DNA into host cells, microparticles of metal are used.

Select the correct statement from the following

[Re-NEET 2020]

The sequence that controls the copy number of the linked DNA in the vector, is termed

[NEET 2020]

The specific palindromic sequence which is recognized by EcoRI is:

Which of the following can act as molecular scissors?

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:

[NEET 2021]

Identify the wrong statement with regard to restriction enzymes.

[NEET 2020]

Plasmid pBR322 has a Pst I restriction enzyme site within gene amp R that confers ampicillin resistance. If this enzyme is used for inserting a gene for Beta-galactoside production and the recombinant plasmid is inserted in an E.coli strain

[NEET 2021]