During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

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YESPYQ · Accepted Answer

Correct answer: b) Denaturation. Polymerase chain reaction (PCR) involves 3 steps: denaturation, annealing and elongation. The denaturation process requires high temperature of about . DNA fragments are joined with the help of DNA ligase enzyme. During annealing, primers bind to the target DNA sequences and initiate polymerisation. This process is performed at . Addition of nucleotides to the primer, synthesises a new DNA with the help of enzyme DNA polymerase. This step is called elongation which is performed at .

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then wh…

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