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Plasmid pBR322 has PstI restriction enzyme site within gene that confers ampicillin resistance, If this enzyme is used f…

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Plasmid pBR322 has PstI restriction enzyme site within gene that confers ampicillin resistance, If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain
aThe transformed cells will have the ability to resist ampicillin as well as produce - galactoside.
bIt will lead to lysis of host cell.
cIt will be able to produce a novel protein with dual ability.
dIt will not be able to confer ampicillin resistance to host cell.
✓ Correct answer: d) It will not be able to confer ampicillin resistance to host cell.
ExplanationCorrect answer: Option D Explanation: In plasmid pBR322, the PstI restriction site lies within the ampicillin resistance gene (ampR).When a foreign gene (here, the gene for β-galactoside production) is inserted at this site, it disrupts the ampR gene.This process is called insertional inactivation.As a result: The recombinant plasmid cannot express ampicillin resistance.The transformed E. coli cells will not survive on ampicillin-containing medium, even though they may produce β-galactoside.Therefore, the correct option is D: It will not be able to confer ampicillin resistance to host cell.
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