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The product(s) of reaction catalyzed by nitrogenase in root nodules of leguminous plants is/are [NEET 2020]

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The product(s) of reaction catalyzed by nitrogenase in root nodules of leguminous plants is/are

[NEET 2020]

a

Ammonia and oxygen

b

Ammonia and hydrogen

c

Ammonia alone

d

Nitrate alone

✓ Correct answer: b)

Ammonia and hydrogen

Explanation
  • In the root nodules of leguminous plants, the enzyme nitrogenase is responsible for biological nitrogen fixation.

  • It converts atmospheric nitrogen gas (N₂) into ammonia (NH₃) using energy from ATP and reducing power from ferredoxin or other sources.

The overall reaction catalyzed by nitrogenase is:

\({\text{N}}_{2}+8{H}^{+}+8{e}^{−}+16ATP\to 2N{H}_{3}+{H}_{2}+16ADP+16Pi\)

  • As you can see, hydrogen gas (H₂) is also produced as a byproduct along with ammonia.

  • Oxygen is not a product — in fact, nitrogenase is highly sensitive to oxygen and works in an anaerobic environment maintained by leghemoglobin in root nodules.

Final Answer: B. Ammonia and hydrogen

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