🏫 Board🧬 Biology

Biotechnology - Principles and Processes

6 Board Biology previous year questions on Biotechnology - Principles and Processes — options free on every question; 1 include the answer & explanation free, the rest unlock with PYQ Pass.

Q1 FREE PREVIEW

Spooling is:

a

Cutting of separated DNA bands from the agarose gel

b

Transfer of separated DNA fragments to synthetic membranes

c

Collection of isolated DNA

d

Amplification of DNA

✓ Correct answer: c)

Collection of isolated DNA

Explanation

During DNA extraction, the ethanol can be gently pushed down into the DNA solution by using a glass rod or wooden spooling stick. The charged DNA molecules move away from the ethanol and can be scooped up and swirled around a glass rod or wooden spooling stick. This is call DNA spooling. DNA spooling can isolate long strands of DNA from a solution.

Q2

First discovered restriction endonuclease was:

a

Pvu I

b

Pst I

c

Hind II

d

EcoRI

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Q3

Main steps in the formation of recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA from the cell.
D. Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:

a

C\( \rightarrow \) A\( \rightarrow \) B\( \rightarrow \) D

b

C\( \rightarrow \) B\( \rightarrow \) D\( \rightarrow \) A

c

B\( \rightarrow \) D\( \rightarrow \) A\( \rightarrow \) C

d

B\( \rightarrow \) C\( \rightarrow \) D\( \rightarrow \) A

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Q4

Thermostable DNA polymerase has been isolated from

a

Agrobacterium tumifaciens

b

Thermus aquaticus

c

Escherichia coli

d

Bacillus thuringiensis

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Q5

Plasmids are extra-chromosomal genetic material of which of the following ?

a

Viruses

b

Algae

c

Fungi

d

Bacteria

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Q6

In case of Bam HI, 'H' represents

a

Genus

b

Species

c

Name of the scientist

d

Strain

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